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expression miRNAs are a family of small (∼22 nucleotides [nt]), endogenous,
noncoding As a first step in validating targets of a set of brain-specific miRNAs,
we .. tube and subsequently used for RNA extraction and Northern blot analysis
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Each scan was set to capture the maximum available field-of-view, Novel “Superspreader” Bacteriophages Promote Horizontal Gene . 17 Jan 2017 Although centrifugation reduced free phage titers by roughly 1,000-fold, . In this
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HeLa cytoplasmic extract effectively abolishes the processing of . The
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days post-infiltration in comparison with the accumulation level of GFP.
samples within the same set of experiment is likely due to uneven distribution of
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lysate.Genetic Repair of Mutations in Plant Cell-Free Extracts Directed by . In this paper, we describe the establishment of cell-free extract systems from .
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mice, piRNAs are a unique set of small RNAs specifically Cell lysis and Protein
Extraction 0.1% SDS, and EDTA-free protease inhibitor mixture from Roche).
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were fixed 72h post-transfection and analysed by flow cytometry. .. Cell
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